Our work on heat-triggered remote control of CRISPR-dCas9 for tunable transcriptional modulation was published in ACS Chemical Biology! Congratulations to Lena and the rest of the team! Read the full manuscript here.
Summary | CRISPR-based approaches have achieved wide success in modulating gene activity to control cell function and are potential tools for clinical therapies. However, the lack of precise methods to deliver and control Cas protein expression in vivo remains a critical hurdle. Here we develop a tunable, heat-triggered platform that regulates mammalian cell transcription by remote control of dCas9 transcriptional modulators.